MICROELECTRODES - Dissertations.se


Parodontit - Käypä hoito

Paul Savelkoul. Edwin Winkel. A. Van Winkelhoff. K. Boutaga.

Subgingival plaque sampling

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Aggregatibacter actinomycetemcomitans had been detected subgingivally in all prior to anti-infective therapy (AT) and METHODS: In 100 patients with aggressive/chronic periodontitis, subgingival plaque was sampled from the deepest pockets per quadrant (MT4) and per sextant (MT6). Plaque samples were taken using two sterile paper points simultaneously. One paper point from each pocket was pooled with the three other paper points of the pockets (MT4). Two Subgingival Plaque‐Sampling Strategies Used With RNA Probes Diana‐Maria Krigar Section of Periodontology, Department of Conservative Dentistry, Clinic for Oral, Dental, and Maxillofacial Diseases, University Hospital Heidelberg, Heidelberg, Germany.

Each participant received a  Periodontal parameters were recorded and deoxyribonucleic acid was extracted from the subgingival plaque and coronary atherosclerotic plaque samples of the   The subgingival plaque samples will be analyzed to define the microbiotic profiles of the patients and the CGF determined to define their inflammatory  The amount of stannous fluoride in GCF and subgingival plaque samples will be analyzed.. Registret för kliniska prövningar. ICH GCP. H2S production was measured with the bismuth method and subgingival plaque samples were analyzed for the presence of 20 bacterial species with the  Subgingival plaque samples are also collected for microbiological analysis of the microbiota with focus on hydrogen sulfide producing bacteria.

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Background: The collection of subgingival plaque samples with paper points is time-consuming and accident-sensitive. However, the collection of saliva  22 Mar 2017 Material and methods: Plaque samples of 50 patients with generalized severe chronic periodontitis before therapy were pooled in two separate  Supragingival plaque should be removed with cotton pellets or periodontal scalers to secure noncontaminated subgingival specimens. Blood and pus at the   gingivalis in saliva and in subgingival plaque samples, showing comparable specificity to culture and real-time PCR. Conclusion: We applied the FRET technology  2 Jan 2018 DNA was then extracted from plaque samples and analyzed by 16S. rRNA gene sequencing.

Klinisk prövning på Subgingival Plaque: stannous fluoride

Subjects will have up to 10 sites identified as "sampling sites" that will have supragingival plaque, subgingival plaque, and gingival crevicular fluid (GCF) collected at Baseline, 30 minutes, 24 hours, and Week 2. Previous studies have demonstrated that the hydrolysis of the trypsin substrate N-benzoyl-DL-arginine-2-naphthylamide (BANA), by subgingival plaque obtained from a single site, correlates best with the numbers and proportions of spirochetes in plaque samples and may serve as an indicator of clinical disease. Two Subgingival Plaque Sampling Strategies Used With RNA-Probes Language: English Authors: Dr. med. dent. Diana Krigar, Dr. med. dent.

Subgingival plaque sampling

T. forsythia, P. nigrescens and C. rectus in the sub-gingival and atherosclerotic plaque samples suggesting that the micoorganisms in the subgingival plaque  The objective of this study was to determine whether the supragingival plaque samples of severe-ECC (S-ECC) patients harbor more EBV load than the  plaque samples suggesting that the micoorganisms in the subgingival plaque had significant chance of their presence in atherosclerotic plaque as well  Microbial analysis of subgingival plaque samples compared to that of Haririan, Hady; Andrukhov, Oleh; Bertl, Kristina; Lettner, Stefan; Kierstein, Sonja; Moritz,  Sugar-free chewing gum helps neutralise plaque acids. Dental plaque samples from monkeys (Macaca fascicularis) were shown to contain proline reduction  av INIC UNIT · Citerat av 5 — samples.
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Thirty patients (18 female) suffering from aggressive (n = 12) or generalised severe chronic (n = 18) periodontitis were included.

E-mail address: nickles@med.uni‐frankfurt.de 1994-08-01 Comparison of Subgingival Bacterial Sampling With Oral Lavage for Detection and Quantification of Periodontal Pathogens by Real-Time Polymerase Chain Reaction.
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Two sterile paper points were inserted simultaneously into the periodontal pockets. Curette sampling and a subgingival washing technique were compared within and between paired sites on two separate occasions in 24 patients using darkfield microscopy. Significantly lower mean proportions of spirochetes and motile rods and higher proportions of non‐motile organisms were obtained with subgingival washing compared with curette sampling. The presence of specific bacteria in subgingival plaque has been used as an indicator of active periodontal disease. The technique of subgingival sampling may conjecturally influence the identification and enumeration of microorganisms reported.